Journal: Cell
Article Title: A white-box machine learning approach for revealing antibiotic mechanisms of action
doi: 10.1016/j.cell.2019.04.016
Figure Lengend Snippet: (A) Overall experimental design for measuring metabolite effects on antibiotic lethality. Overnight cultures of E. coli MG1655 were inoculated into MOPS minimal medium, grown to early exponential phase, and back-diluted to OD600 = 0.1. Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 (Bochner, 2009) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added. OD600 was measured after 4 hours of incubation at 37°C and 900 rpm shaking. Antibiotic IC50s were estimated for each antibiotic-metabolite combination.
Article Snippet: Cells were dispensed into Biolog phenotype microarray plates (PMs) 1–4 ( Bochner, 2009 ) with different concentrations of ampicillin (AMP), ciprofloxacin (CIP) or gentamicin (GENT) added.
Techniques: Microarray, Incubation